Food Hydrocolloids for Health · Published 2025-10-11 · DOI 10.1016/j.fhfh.2025.100248
Polygonati Rhizoma serve as medicinal and edible plants rich in carbohydrates, with its polysaccharides extensively studied, while research on oligosaccharides remains limited regarding their structural and bioactive properties. This study investigates the against non-alcoholic fatty liver disease (NAFLD) effects of Polygonatum kingianum oligosaccharides (PKO) and employs UPLC-Orbitrap-MS/MS technology to characterize oligosaccharide components. The PKO components were enriched through hot water extraction, 60% anhydrous ethanol precipitation, and membrane separation technology, with preliminary validation of their hepatoprotective effects in DL-ethionine-induced fatty liver mice. A novel LC-MS/MS analytical method was developed utilizing characteristic secondary fragment ions and retention time profiles of oligosaccharides to enable rapid identification and characterization of PKO. Thirty-four oligosaccharides were successfully identified from Polygonatum kingianum (PK), all exhibiting a monosaccharide composition of fructose and glucose. The structural backbone consists of fructose residues linked via (1→2) and (2→6) glycosidic bonds, with 32 compounds being reported for the first time in PK. This study marks the first discovery of oligosaccharides in PK with potential therapeutic effects against NAFLD. Additionally, it represents the first application of LC-MS/MS technology to systematically characterize neutral oligosaccharides in this species. These findings provide a novel analytical methodology for investigating potential anti-NAFLD oligosaccharide components in PK.
Abstract from DOAJ. Public domain (CC0 1.0).
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