Exploratory Animal and Medical Research · Published 2025-01-01 · DOI 10.52635/eamr/15.1.100-106
The present study was carried out to detect the causative agent of sudden death in guinea fowl f lock maintained at organized poultry farm, Orathanadu. On post mortem examination, liver was enlarged and revealed multiple grayish areas. The spleen was enlarged and kidneys were pale and swollen. Breast muscle was very thin and the keel bone was prominent. Representative tissue samples from liver, kidney spleen were collected for histopathological examination and revealed lymphoid and reticuloendothelial cells in kidney, liver and spleen. PCR was used for detection as well as differentiation of avian neoplastic viruses such as Marek’s disease virus, avian leukosis complex and reticuloendotheliosis virus using specific primer sets. The sample was found to be positive for avian reticuloendotheliosis virus and negative for Marek’s disease virus and avian leukosis complex. The positive PCR amplicons were subjected to nucleotide sequencing for further confirmation of avian reticuloendotheliosis virus. The sequence analysis showed 99% homology with other published REV isolates available in the NCBI database. The present study reported the first incidence of avian reticuloendotheliosis virus in guinea fowl flock based on gross as well as histopathological lesions and molecular methods in India.
Abstract from DOAJ. Public domain (CC0 1.0).
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