Exploratory Animal and Medical Research · Published 2025-01-01 · DOI 10.52635/eamr/15.2.188-195
Porcine circovirus type 2 (PCV2) is a major causative agent of porcine circovirus-associated disease (PCVAD), responsible for significant financial losses in the swine sector. Despite the availability of commercial vaccines globally, India lacks an affordable indigenous PCV2 vaccine, with most preventive immunization dependent on imported formulations. In this study, we developed and characterized a novel chimeric PCV1-2 construct by fusing the replication-associated ORF1 of non-pathogenic PCV1 with the capsid-encoding ORF2 of PCV2. The rescued recombinant virus was successfully propagated in a subclone of porcine kidney cells, demonstrated productive replication across passages, as evidenced by gene-specific PCR, increasing capsid antigen detection by indirect ELISA (one-way ANOVA, p<0.05), immunofluorescence, and partial sequencing of ORF1/ORF2 confirmed the expected chimeric identity. These in vitro data indicate that the PCV1–2 construct is functional in porcine cells and suitable for further evaluation as a basis for developing an indigenous, low-cost approach to PCV2 control.
Abstract from DOAJ. Public domain (CC0 1.0).
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