Exploratory Animal and Medical Research · Published 2026-06-01 · DOI 10.52635/eamr/16.1.77-84
Arya Mahesh Narkhede, Satya Nidhi Shukla, Shivika Chouksey, Anand Kumar Jain
The freezing and thawing process during cryopreservation of semen decreases motility, compromises membrane integrity and impairs cell function, along with the fertilizing capacity of the spermatozoa. The present study aimed to evaluate the efficacy of platelet-rich plasma (PRP) as a supplement in semen extenders to improve post-thaw fertility of Barbari and Sirohi buck semen. For this study, 288 ejaculates were collected from both breeds, of which 30 best ejaculates from each breed were processed and cryopreserved using a standard protocol. Each ejaculate was divided into three equal aliquots. A tris egg-yolk citrate extender was utilized for dilution, to which varying percentages of PRP were incorporated. The first aliquot of semen was diluted with extender containing 0% PRP (G-I), the second with extender containing 7% PRP (G-II) and the third with extender containing 10% PRP (G-III), respectively. All the ejaculates were evaluated at pre-freeze, equilibration and post-thaw stages. At the pre-freeze stage, semen parameters did not vary significantly across different extenders. However, significantly higher (p<0.05) percentage of progressive motility (54.00±1.54,53.66±1.49), sperm viability (62.73±1.54,58.36±1.44) and plasma membrane integrity (51.63±1.85,50.23±2.43) was recorded during post post-thaw stage in Barbari and Sirohi bucks extender supplemented with 7 % PRP. The inclusion of 7% PRP enhanced post-thaw fertility and had a beneficial impact on the semen freezability of Barbari and Sirohi bucks.
Abstract from DOAJ. Public domain (CC0 1.0).
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Narkhede, A., Shukla, S., Chouksey, S., et al. (2026). THE EFFECT OF PLATELET-RICH-PLASMA SUPPLEMENTED EXTENDER ON POST-THAW FERTILITY OF SIROHI AND BARBARI BUCK SEMEN. Exploratory Animal and Medical Research. https://doi.org/10.52635/eamr/16.1.77-84