Medical Journal of Dr. D.Y. Patil Vidyapeeth · Published 2026-07-01 · DOI 10.4103/mjdrdypu.mjdrdypu_526_25
Sinduja Subramaniyan, C. Umesh Kumar
Background: Non-fermenting gram-negative bacilli (NFGNB) are important opportunistic pathogens in hospital settings. Their ability to acquire carbapenemase genes contributes to high levels of antimicrobial resistance and limited treatment options. Aims: To study the prevalence, antimicrobial resistance patterns, and molecular detection of carbapenemase-encoding genes in clinical isolates of NFGNB. Settings and Design: A prospective, cross-sectional study conducted at a tertiary care hospital microbiology laboratory. Material and Methods: Two hundred NFGNB isolates from various clinical specimens were identified using standard biochemical tests. Kirby determined antimicrobial susceptibility–Bauer disc diffusion and MIC testing as per CLSI guidelines. Carbapenem-resistant isolates were screened for Metallo-β-lactamase production and subjected to polymerase chain reaction (PCR) for detection of carbapenemase genes (blaIMP, blaVIM). Statistical Analysis Used: Descriptive statistics were applied. Chi-square test was used to analyze associations between resistance profiles and gene detection. Results: Pseudomonas aeruginosa and Acinetobacter baumannii were the predominant species isolated. A high prevalence of multidrug resistance was observed, particularly resistance to imipenem. PCR confirmed the presence of blaIMP and blaVIM genes in several carbapenem-resistant isolates, correlating with Metallo-β-lactamase activity. Conclusions: NFGNB exhibit significant multidrug and carbapenem resistance. Molecular detection of carbapenemase genes emphasizes the need for routine genetic surveillance to support antimicrobial stewardship and effective infection control.
Abstract from DOAJ. Public domain (CC0 1.0).
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Subramaniyan, S., Kumar, C. (2026). Genomic Clues and Resistance Blues: Tracking non-fermenting Gram-negative Bacilli in Clinical Practice. Medical Journal of Dr. D.Y. Patil Vidyapeeth. https://doi.org/10.4103/mjdrdypu.mjdrdypu_526_25