Comparative Immunology Reports · Published 2026-04-14 · DOI 10.1016/j.cirep.2026.200280
As an important enzyme catalyzing histone deacetylation, histone deacetylase 11 (HDAC11) can modulate chromatin accessibility and play important roles in the transcriptional regulation, which further regulates many physiological processes including immune memory and embryo development in vertebrates. In the present study, a histone deacetylase (designated CgHDAC11) was identified from the Pacific oyster Crassostrea gigas. The CgHDAC11 gene contained an open reading frame of 1,167 bp and was predicted to encode a polypeptide of 388 amino acids with a conserved classical deacetylase domain. Sequence identity analysis revealed that the deduced enzyme shared high similarity with the previously identified HDAC11 from other species. The total acetylation level of histone H3 in CgHDAC11 transfected HEK293T cells decreased significantly (p < 0.05). In C. gigas, the mRNA expression of CgHDAC11 was highly expressed in adult tissues with much higher expression level in hepatopancreas and haemocytes. By immunofluorescence, the protein of CgHDAC11 was found to distribute in both nucleus and cytoplasm of haemocytes. The mRNA expression of CgHDAC11 in haemocytes was promptly up-regulated at 3 h and 6 h (p < 0.05) after Vibrio splendidus stimulation. When the oysters were pretreated with inactivated V. splendidus, much higher expression of CgHDAC11 was detected in the second immune response against V. splendidus, which was 3.02-fold (p < 0.05) of that in the first response. After CgHDAC11 was inhibited by specific inhibitor FT895 in primed oysters, the expressions of CgIL17-1 and CgSOCS6 in haemocytes were increased significantly post V. splendidus second stimulation (p < 0.05), while that of CgIL17-5 was significantly down-regulated (p < 0.05). These results indicated that the oyster CgHDAC11 was a conserved member of HDAC family and was involved in immune priming by regulating the production of immunoregulatory molecules, which provided a new idea about the epigenetic regulation in immune response of invertebrates.
Abstract from DOAJ. Public domain (CC0 1.0).
Read the article at the publisher →