Comparative Immunology Reports · Published 2025-10-01 · DOI 10.1016/j.cirep.2025.200253
Long non-coding RNA (lncRNA) is a class of non-coding RNA defined by a length of exceeding 200 nucleotides. Evidence suggests that immune-related lncRNAs played crucial roles in fish immune regulation. The black rockfish (Sebastes schlegelii) is an important marine aquaculture fish in northern China with significant economic value. Aeromonas salmonicida, a Gram-negative bacterium, causes surface ulceration, intestinal congestion, and kidney enlargement in infected fish. Nevertheless, how lncRNAs effect immune regulation after bacterial infection remains poorly characterized. Accordingly, the aim of this study was to identify lncRNAs in the liver of A. salmonicida infected S. schlegelii. Briefly, we identified a total of 6204 lncRNAs and predicted 32,158 co-expression and 47,244 co-location lncRNA-mRNA pairs. A total of 739 lncRNAs exhibited altered expression patterns, with the number of upregulated lncRNAs slightly exceeding that of downregulated ones. GO enrichment and KEGG pathway analyses of differentially expressed mRNAs (DE-mRNAs) revealed their involvement in the PPAR signaling pathway, adipocytokine signaling pathway, phosphorylation, acute-phase response, and acute inflammatory response, suggesting lncRNAs’ crucial roles in regulating mRNA expressions related to signal transduction, immune signaling pathways and intracellular immune responses. Furthermore, we randomly selected DE-mRNAs and lncRNAs for qRT-PCR analysis to confirm the accuracy of the transcriptome data. These findings provide novel insights into the role of lncRNAs in the immune response of rockfish and serve as valuable resources for further research on the roles of lncRNAs in teleost pathogen infections.
Abstract from DOAJ. Public domain (CC0 1.0).
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