Child's Health · Published 2025-12-01 · DOI 10.22141/2224-0551.20.7.2025.1921
Background. Hirschsprung’s disease is one of the most common neonatal obstruction diseases. The annual cases of Hirschsprung’s disease in Indonesia are approximately 1,400 live births. Its screening and diagnosis can be established using several methods, but they have various limitations; therefore, alternative methods are required. Patients with Hirschsprung’s disease tend to have higher acetylcholinesterase (AchE) levels, and it is potentially applied as an alternative diagnostic examination for this condition. The aim was to further determine AchE levels in patients with Hirschsprung’s disease. Materials and methods. This prospective cohort study was conducted in Dr. Soetomo Regional General Hospital, Surabaya, from November 2024 to January 2025. The demographic, surgery, and pre- and post-resection AchE levels data were collected. The Human AChE (Acetylcholinesterase) ELISA Kit (Elabscience E-EL-H6031) was used to assess the AchE levels. The collected data were analysed using the chi-Square test, the pre- and post-resection AchE levels were analysed using a paired t-test. A p-value < 0.05 was considered statistically significant. Results. The study included 6 participants. Most of them were male (83.3 %) with a mean age of 34.830 ± 34.143 months. The analysis revealed a significant difference in AchE levels pre- and post-resection (299.875 and 85.267 units/mL, p-value of 0.014) with a very strong relationship (Cohen’s d effect size 1.250). Conclusions. Acetylcholinesterase levels will decrease after surgery. Evaluation of AchE content may be potentially used as a diagnostic and prognostic examination for Hirschsprung’s disease.
Abstract from DOAJ. Public domain (CC0 1.0).
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