Frontiers in Immunology · Published 2026-08-05 · DOI 10.3389/fimmu.2026.1849622
Jennifer Fraszczak, David Obwegs, Kaifee Arman, Tanja Muralt, Bavanitha Thurairajah, Ève Mallet Gauthier, Irah L. King, Heather J. Melichar, Sagar, Tarik Möröy
GFI1 is a DNA-binding transcription factor that regulates the commitment of hematopoietic precursors to myeloid and lymphoid lineages. Here we report that GFI1 is expressed in γδ T cells and restricts the cellularity of RORγt+Vγ6+ γδ T cells that produce high levels of IL-17A while promoting the expansion of Vγ1+ and Vγ4+ γδ T cells. GFI1 deficiency results in an expansion of Vγ6+ γδT17 cells that starts post-birth and is strongly exacerbated upon challenge with an inflammatory allergen. Additionally, we observe an expansion of RORγt+/MAF+ cells within the thymic DN1e population of GFI1-deficient mice. The DN1e population, along with other DN subsets in GFI1-deficient mice, exhibits a distinctive γδT17 cell-specific transcriptomic profile. Specifically, DN1, DN3, and γδ T cells lacking GFI1 show upregulation of the B-ZIP transcription factor MAF, which regulates genes critical for γδ T cells, such as IL-17a, IL-22, and Blk. The Maf gene is occupied by GFI1 in DN pre-T cells at cognate binding sites in its promoter region, suggesting that GFI1 acts as a direct repressor of Maf. We conclude that GFI1 functions as a novel regulator of Vγ6+ γδT17 precursor cells restricting their peripheral expansion by acting upstream of a MAF-dependent regulatory network.
Abstract from DOAJ. Public domain (CC0 1.0).
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Fraszczak, J., Obwegs, D., Arman, K., et al. (2026). GFI1 as a novel regulator of γδ T cell development and the IL-17/IFN-γ lineage commitment. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2026.1849622