Annals of Microbiology · Published 2026-05-29 · DOI 10.1186/s13213-026-01860-y
Yang Li, Haotian Ma, Ruixue Pan, Yuner Long, Yining Zhao, Jinju Peng, Fucheng Guo, Yuexia Ding, Yi Ma
Abstract Background Bacteriocins are peptides that demonstrate antimicrobial activity and are produced by bacteria. Bacillus velezensis is considered a reliable producer of bacteriocins, with a strong antimicrobial spectrum and antimicrobial activity. In this study, B. velezensis G7 isolated from mangrove plant roots was used as the experimental strain. Whole-genome sequencing (WGS) analysis was performed to annotate functional genes. Results The results demonstrated that B. velezensis G7 has a genome size of 3,894,836 bp, a GC content of 46.67%, and predicts 3,715 CDSs, 10 rRNA genes, and 83 tRNA genes. Gene annotation revealed pathways related to bacteriocin synthesis and immunity. Identified 8 bacteriocin and secondary metabolite biosynthetic gene clusters in B. velezensis G7, including Sactipeptides, LCI, Amylocyclicin, ComX4, Mersacidin, Lanthipeptide, Bacilysin, and NRPS. The crude protein from B. velezensis G7 shows broad-spectrum antibacterial activity, with the strongest inhibition against Listeria monocytogenes and the weakest against Staphylococcus aureus. Conclusions The genome of B. velezensis G7 was analyzed, and bacteriocin-related genes were identified. The results suggest that B. velezensis G7 has the potential to produce bacteriocins, thereby providing a theoretical basis for future studies on bacteriocin production in B. velezensis. In addition, these findings may facilitate further advances in bacteriocin research and bioinformatics analysis.
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Li, Y., Ma, H., Pan, R., et al. (2026). Whole genome sequencing and analysis of the bacteriocin-producing gene in Bacillus velezensis G7. Annals of Microbiology. https://doi.org/10.1186/s13213-026-01860-y