Stem Cell Research & Therapy · Published 2026-08-05 · DOI 10.1186/s13287-026-05214-y
Michael Singh, Karina Zitta, Thilo Wedel, Ralph Lucius, François Cossais, Peter Behrendt, Sabine Fuchs, Jan-Tobias Weitkamp, Rouven Berndt, Olga Zimmermann, Tim Schricker, Andreas Bayer, Martin Albrecht
Abstract Platelet-Released Growth Factors (PRGF) are a standardized, cell- and fibrin-free platelet lysate with translational potential for tissue repair. PRGF enhance tissue regeneration, particularly in contexts where angiogenesis is critical for restoring perfusion in chronic and non-healing wounds. Here, we present the first in vitro study demonstrating that PRGF exert pro-angiogenic effects at a working concentration of 10%. The study employs an endothelial cell-based model integrating targeted gene expression analysis, array-based profiling of angiogenesis-related proteins, and functional endothelial tube formation assays. In this setting, screening-level transcriptional and proteomic profiling indicated higher transcript levels of angiogenesis-related genes and increased signals of representative angiogenesis-associated mediators (e.g., Platelet Factor 4, Angiopoietin-1, Angiogenin) following PRGF stimulation. Functional tube formation assays demonstrated significant enhancement of multiple pro-angiogenic parameters in response to PRGF, including number of pieces (P < 0.001), junctions (P < 0.01), and segments (P < 0.05). These findings suggest that 10% PRGF may support angiogenesis and tissue regeneration and provide a foundation for further validation in more complex multicellular systems and in vivo models.
Abstract from DOAJ. Public domain (CC0 1.0).
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Singh, M., Zitta, K., Wedel, T., et al. (2026). Platelet-released growth factors promote pro-angiogenic effects in endothelial cells: a translational perspective on wound healing and regenerative medicine. Stem Cell Research & Therapy. https://doi.org/10.1186/s13287-026-05214-y