Bangladesh Journal of Pharmacology · Published 2026-06-26 · DOI 10.3329/bjp.v21i2.88473
Liming Wu, Xia Jiang, Haoyun Zhu, Rong Zhou, Xiaoliang Xiang
Sinomenine, a bioactive alkaloid from Sinomenium acutum, exhibits anti-inflammatory and neuroprotective effects, but its role in neuronal differentiation and morphological maturation remains unclear. This study used Neuro-2a cells and primary neural stem/progenitor cells (NSPCs) to examine the pro-differentiation effects of sinomenine. Sinomenine increased Neuro-2a cell viability at concentrations of 0.1–20 μM and showed no detectable cytotoxicity up to 100 μM. Under differentiation conditions, sinomenine enhanced neuronal differentia-tion and neurite outgrowth in Neuro-2a cells dose-dependently (5–50 μM), increased the proportion of β-tubulin III–positive neurons, promoted multi-neurite formation, and enhanced dendritic comple-xity in NSPCs. Mechanistically, sinomenine selectively induced Akt phosphorylation without activating ERK, p38, or JNK pathways, and pharmacological inhibition of Akt abolished sinomenine-induced neuritogenesis. These findings suggest sinomenine promotes neuronal differentiation and neurite outgrowth via the Akt pathway, supporting its potential in neuroregeneration.
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Wu, L., Jiang, X., Zhu, H., et al. (2026). Sinomenine promotes neuronal differentiation and neurite out-growth via Akt activation in vitro. Bangladesh Journal of Pharmacology. https://doi.org/10.3329/bjp.v21i2.88473