Frontiers in Toxicology · Published 2026-07-20 · DOI 10.3389/ftox.2026.1839932
Manon Dussol, Sandrine Ménard, Margaux Papin, Maeva Boulée, Vérane Bard, Melvin Airaud, Daphna Fenel, Christine Moriscot, Frédérick Barreau, Marie Carriere
The daily use of plastic products leads to exposure of populations via ingestion, which may affect intestinal health. While a wealth of studies has documented the impact of micro- and nanoplastics (MNPLs) on models representing healthy human intestine, less is known about their impact on sensitive populations, particularly those at risk of developing inflammatory bowel diseases. This study explored the intestinal response to carboxylated and aminated polystyrene particles (50–500 nm PS-COOH and 50 nm PS-NH2) in vivo and in vitro in models of genetic susceptibility to Crohn’s disease. These models included nucleotide-binding oligomerization domain-2 wild-type and knock-out mice (Nod2WT and Nod2KO, respectively) and co-cultures of Caco-2 and HT29-MTX cells, with Caco2 cells stably expressing either wild-type NOD2 or the NOD21007fs variant, which is strongly associated with Crohn’s disease. In vitro, the co-cultures were used either in a non-differentiated state (i.e., 24 h post-seeding) or after differentiation into a tight, mucus-secreting epithelium. Results showed that in vivo, single oral administration of PS-COOH particles impaired the paracellular permeability of the ileum of Nod2WT mice. Both PS-COOH and PS-NH2 reduced the number of goblet cells in Nod2WT and Nod2KO mice as well as Muc4 secretion in Nod2WT mice, and PS-COOH reduced Muc4 secretion in Nod2KO mice. Single oral administration of PS-COOH or PS-NH2 altered mRNA expression of genes involved in the production of antimicrobial peptide, inflammation, mucin secretion and endoplasmic reticulum stress pathways, in both Nod2WT and Nod2KO mice. In vitro, PS-COOH showed no impact in Caco2-NOD21007fs/HT29-MTX and in Caco2-NOD2WT/HT29-MTX, whereas PS-NH2 induced a similar toxic response in both cell systems. The intensity of this response depended on the cells’ differentiation status and on the mode of exposure, i.e., acute versus repeated. Overall, this study explored, for the first time, the response of models of genetic susceptibility to Crohn’s disease to exposure to PS-COOH and PS-NH2 nanoplastics (NPLs). The obtained data provide new insight into how populations at risk of developing CD respond to PS-COOH and PS-NH2 NPLs, i.e., new knowledge related to the impact of NPLs on this vulnerable population.
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Dussol, M., Ménard, S., Papin, M., et al. (2026). Intestinal toxicity of functionalized polystyrene nanoplastics in NOD2 models of Crohn’s disease susceptibility. Frontiers in Toxicology. https://doi.org/10.3389/ftox.2026.1839932