Dentistry Journal · Published 2026-07-09 · DOI 10.3390/dj14070422
Kaoru Kobayashi, Ryota Yamasaki, Michihiko Usui, Masahiro Morita, Maki Inoue, Yoshie Nagai-Yoshioka, Masanori Iwasaki, Keisuke Nakashima, Tatsuji Nisihara, Wataru Ariyoshi
<b>Background</b>: <i>Porphyromonas gingivalis</i> is the principal bacterial species implicated in the pathogenesis of periodontitis. Despite its central role, direct detection of periodontopathic bacteria is not routinely performed in dental clinics. Although test kits using monoclonal antibodies against <i>P. gingivalis</i> have been reported, no practical chairside method has yet been established. <b>Objectives</b>: In this study, we developed an immunochromatography system that enables rapid and quantitative detection of <i>P. gingivalis</i> within 10 min. <b>Methods</b>: We evaluated the performance of the developed immunochromatography system and its dedicated measurement reader. <b>Results</b>: The kit demonstrated a minimum detection limit of 2.6 × 10<sup>4</sup> CFU/mL and maintained detection capability across fimbrial types I and II. The dedicated reader showed strong correlations with a reference reader (<i>R</i><sup>2</sup> = 0.9921) and visual color chart evaluation (<i>R</i><sup>2</sup> = 0.9347). High intra- and inter-device precision was observed, with coefficients of variation less than 0.6%. Although the presence of 2% whole blood interfered with visual interpretation, other oral substances, including toothpastes and mouthwashes, did not significantly affect the assay performance. <b>Conclusions</b>: Collectively, these findings indicate that the developed system may serve as a rapid and reliable chairside tool for quantitative detection of <i>P. gingivalis</i>, with potential utility in the diagnosis and management of periodontitis and related systemic conditions.
Abstract from DOAJ. Public domain (CC0 1.0).
Read the article at the publisher →
Kobayashi, K., Yamasaki, R., Usui, M., et al. (2026). Development of a Quantitative Detection System for Porphyromonas gingivalis Based on Immunochromatography. Dentistry Journal. https://doi.org/10.3390/dj14070422