Biomedical Analysis · Published 2024-11-18 · DOI 10.1016/j.bioana.2024.11.002
Circular RNA (circRNA) is a unique type of non-coding RNA, which is produced by back splicing of mRNA to form the covalent junction without 3′-5′ polarity and poly(A) tail. With the development of detection technology, a large number of circRNAs have been identified in organisms, and their biological functions and mechanisms have been uncovered gradually. In particular, circRNAs are abnormally expressed and play a critical role in many diseases, including gastric cancer, hepatocellular carcinoma, breast cancer, etc., showing great potential as disease biomarkers. At present, the main methods for detecting circRNA include RNA sequencing, RT-PCR, Northern blotting, etc., which have limited sensitivity and specificity. Recently, some new methods, such as isothermal amplification and digital droplet platform, have emerged to improve the sensitivity and quantitative accuracy for circRNA detection. In this review, we summarized the traditional and emerging methods for circRNA detection as well as the advantages and limitations, and look forward to future research directions.
Abstract from DOAJ. Public domain (CC0 1.0).
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