Journal of Clinical Virology Plus · Published 2025-12-23 · DOI 10.1016/j.jcvp.2025.100240
Introduction: Blood transfusion, although lifesaving, may carry inherit risks, including transfusion-transmitted infections (TTIs). This study aims to assess the prevalence and characteristics of HBV infection, including occult HBV and other TTIs (HCV, HIV and syphilis) among blood donors in Albania. Methods: This retrospective observational study analysed blood donor samples from the National Blood Transfusion Center in Tirana (January–December 2024). Serological assays and nucleic acid testing (NAT) were performed following standard protocols. All donations positive for HBsAg, HCV, syphilis, HIV, NAT triple assay, or with ALT >2 × ULN were included. Demographic and clinical data collected comprised age, gender, region, education level, blood type, BMI, and ALT values. Results: Among 25,474 donors, 6.4 % tested positive at the first screening for at least one infection marker or liver injury indicator. Most positive cases were male (82 %), with a median age of 40 years, and had secondary education (42 %). Serology identified HBsAg 2.8 %, anti HCV 0.6 %, HIV 0.1 % (confirmed positive 0.057 %), and syphilis 0.4 % (confirmed positive 0.15 %). Overall, a prevalence of 3.1 % for confirmed active infections (HBV, HCV, HIV and syphilis) was found. NAT triple assay was reactive in 860 samples; 65 HBsAg-negative cases (0.2 %) were confirmed as HBV-DNA positive (occult HBV infection, OBI). OBI cases had a median anti-HBc and anti-HBs titers of 6.3 and 7.3 respectively, with 40 % showing positive anti-HBs levels. No significant demographic or clinical differences were found between anti-HBs positive and negative individuals. In multivariable analysis, lack of vaccination, lower education, and lower ALT remained independent predictors of HBV infection. Conclusions: This study emphasizes the importance of comprehensive screening strategies, including NAT, to identify occult infections that could compromise blood safety. Continued promotion of vaccination, education, and regional surveillance is essential to reduce the burden of HBV and other TTIs in blood donors and the general population.
Abstract from DOAJ. Public domain (CC0 1.0).
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