Acta Clinica Croatica · Published 2025-01-01 · Journal article · DOI 10.20471/acc.2025.64.03.13
The purpose of this study was to investigate the expression of the ADIPOQ and transcription factor 7-like 2 (TCF7L2) genes in type 2 diabetes mellitus (T2D) in a Pakistani population. This aim of the study was to find the potential link between the expression of the ADIPOQ and TCF7L2 genes in T2D. This cross-sectional study included 200 patients with T2D and 200 control subjects. Analysis of the expression of the ADIPOQ and TCF7L2 genes was performed by real-time polymerase chain reaction. A significant increase in the expression of ADIPOQ and TCF7L2 genes was observed in T2D group as compared to control group. In T2D group, a significant positive relationship was found between the expression of the ADIPOQ gene and systolic blood pressure, random blood glucose and expression of TCF7L2 gene. A significant negative correlation was observed between the expression of the TCF7L2 gene and diastolic blood pressure. Multivariable linear regression analysis revealed that the expression of TCF7L2 gene (β=0.274; adjusted R2 change, 0.135; p=0.005) and expression of ADIPOQ gene (β=0.270; adjusted R2 change, 0.148; p=0.001) were positively associated in T2D, whereas diastolic blood pressure (β=-0.296; adjusted R2 change, 0.148; p=0.001) was negatively associated in T2D as compared to control group. When stepwise linear regression was performed considering the expression of the TCF7L2 gene as a dependent variable in T2D group, one model was computed showing the expression of ADIPOQ gene (β=0.335, p=0.001) as an important predictor of T2D. When stepwise linear regression was employed considering the expression of ADIPOQ gene as a dependent variable, two models were computed showing the expression of TCF7L2 gene (β=0.335; p=0.001) and systolic blood pressure (β=0.138; p=0.041) as important determinators of T2D. Thus, the expression of the ADIPOQ and TCF7L2 genes are independent predictors of T2D. In conclusion, there was increased expression of the ADIPOQ gene and TCF7L2 gene in T2D subjects as compared to controls. The ADIPOQ gene expression and TCF7L2 gene expression provide independent predictors of T2D indicating their role in the development of T2D. Future research is required to elucidate the mechanisms involved in the increased ADIPOQ and TCF7L2 gene expression in T2D.
Abstract from DOAJ. Public domain (CC0 1.0).
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